GlycoSens™ Trehalase (THL) Assay Kit, Micro-method

The GlycoSens™ Trehalase (THL) Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of trehalase (THL) levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Trehalose Series

THL

Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard

Tissues, other biological samples

10 μL - 50 μL

Quantification of trehalase (THL) is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalase

Trehalase; THL

In the context of biological research, trehalase (THL) has emerged as a molecule of high interest. It selectively processes distinct biochemical substrates, maintaining the efficiency of cellular networks. In addition, investigating its functional state is crucial for identifying novel biomarkers and therapeutic targets.

Widely applicable for tracking trehalase (THL) fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Superior Accuracy; Low Background Noise; Highly Stable Signal Output

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.