GlycoSens™ Trehalase (THL) Assay Kit, Spectrophotometry

The GlycoSens™ Trehalase (THL) Assay Kit utilizes a highly stable detection system tailored for accurate profiling of trehalase. It combines streamlined sample handling with superior signal-to-noise ratios to support dependable biochemical assessment in research applications.

Trehalose Series

THL

Reaction Buffer; Specific Substrate; Standard; Reaction Stop Solution

Tissues, other biological samples

10 μL - 50 μL

Target trehalase (THL) in samples undergoes specific enzymatic conversion or chromatic reaction, resulting in a distinct optical absorbance change monitored spectrophotometrically. The variation in absorbance intensity directly correlates with the concentration of target THL.

Within 1 hour

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Trehalase

Trehalase; THL

Trehalase (THL) is an integral element required for robust cellular function and metabolism. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Consequently, consequently, robust measurement of this parameter is highly beneficial for comprehensive functional profiling.

Tailored for accurate quantification of trehalase (THL) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Enhanced Sensitivity; Streamlined Workflow; Consistent Batch-to-Batch Performance

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.