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Quantitative Human 5HTR2A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX345)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human 5HTR2A (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 2A (5HTR2A) levels. 5HTR2A is a G protein-coupled receptor, which binds to serotonin. 5HTR2A plays a role in regulating mood. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.089 ng/mL, which can accurately detect low concentrations of 5HTR2A in the sample.
Target 5HTR2A
N-Glycosylation Site 8, 38, 44, 51, 54
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.089 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name 5-Hydroxytryptamine receptor 2A
Alternate Names 5HTR2A; 5-Hydroxytryptamine receptor 2A; 5-HT2A; Serotonin receptor 2A
Uniprot No. P28223
Application The quantitative human 5HTR2A (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated 5HTR2A levels. It is useful for studying the role of 5HTR2A in serotonin signaling.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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