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Quantitative Human 5HTR2A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX345

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Specifications
Product Description
The quantitative human 5HTR2A (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor 2A (5HTR2A) levels. 5HTR2A is a G protein-coupled receptor, which binds to serotonin. 5HTR2A plays a role in regulating mood. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.089 ng/mL, which can accurately detect low concentrations of 5HTR2A in the sample.
Target
5HTR2A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.089 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor 2A
Alternate Names
5HTR2A; 5-Hydroxytryptamine receptor 2A; 5-HT2A; Serotonin receptor 2A
Uniprot No.
N-Glycosylation Site
8, 38, 44, 51, 54
Application Notes
Application
The quantitative human 5HTR2A (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated 5HTR2A levels. It is useful for studying the role of 5HTR2A in serotonin signaling.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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