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Quantitative Human 5HTR5A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX428

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Specifications
Product Description
The quantitative human 5HTR5A (glycosylated) sandwich ELISA kit is designed to detect human 5-hydroxytryptamine receptor R5A (5HTR5A) levels. 5HTR5A is a G protein-coupled receptor that binds serotonin, which is expressed in the central nervous system. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.082 ng/mL, which can accurately detect low concentrations of 5HTR5A in the sample.
Target
5HTR5A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.082 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
5-Hydroxytryptamine receptor R5A
Alternate Names
5HTR5A; 5-Hydroxytryptamine receptor R5A
Uniprot No.
N-Glycosylation Site
6, 21
Application Notes
Application
The quantitative human 5HTR5A (glycosylated) sandwich ELISA kit is designed to measure glycosylated 5HTR5A levels in samples. This measurement is valuable in neuroscience research, particularly for studying serotonin signaling. The kit is applied in investigations of conditions related to mood disorders.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-95%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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