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Quantitative Human ADRα1A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX382

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Specifications
Product Description
The quantitative human ADRα1A (glycosylated) sandwich ELISA kit is designed to detect human adrenergic receptor α 1A (ADRα1A) levels. ADRα1A is a G protein-coupled receptor, which binds to norepinephrine and epinephrine. ADRα1A plays a role in regulating vasoconstriction. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.151 ng/mL, which can accurately detect low concentrations of ADRα1A in the sample.
Target
ADRα1A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.151 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Adrenergic receptor α 1A
Alternate Names
ADRα1A; Adrenergic receptor α 1A; ADR-A1A; ADRA1L1; Alpha 1a-Adrenoceptor
Uniprot No.
N-Glycosylation Site
7, 13, 22
Application Notes
Application
The quantitative human ADRα1A (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated ADRα1A levels. It is useful for studying the role of alpha-1 adrenergic receptors in blood pressure regulation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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