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Quantitative Human ANGPTL1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX122

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Specifications
Product Description
The quantitative human ANGPTL1 (glycosylated) sandwich ELISA kit is designed to detect human angiopoietin like protein 1 (ANGPTL1) levels. ANGPTL1 is a secreted protein involved in angiogenesis, vascular remodeling, and hematopoietic stem cell regulation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.072 ng/mL, which can accurately detect low concentrations of ANGPTL1 in the sample.
Target
ANGPTL1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.072 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Angiopoietin like protein 1
Alternate Names
ANGPTL1; Angiopoietin like protein 1; ARP1; ANGPT3; Angiopoietin-3
Uniprot No.
N-Glycosylation Site
160, 188
Application Notes
Application
The quantitative human ANGPTL1 (glycosylated) sandwich ELISA kit is valuable for research examining ANGPTL1's role in vascular biology, hematopoiesis, and related diseases, enabling researchers to determine ANGPTL1 concentrations and understand its involvement in these processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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