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Quantitative Human BSP (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX302)

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1×48 T
1×96 T

Product Description The quantitative human BSP (glycosylated) sandwich ELISA kit is designed to detect human bone sialoprotein (BSP) levels. BSP is a major component of the bone extracellular matrix. It's a highly glycosylated protein that plays a crucial role in bone mineralization. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 55 pg/mL, which can accurately detect low concentrations of BSP in the sample.
Target BSP
N-Glycosylation Site 104, 177, 182, 190
O-Glycosylation Site 119, 122, 227, 228, 229, 238, 239
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 55 pg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 150 pg/mL-6000 pg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Bone sialoprotein
Alternate Names BSP; Bone sialoprotein; IBSP; BspII; Integrin-Binding bone sialoprotein; Bone sialoprotein 2
Uniprot No. P21815
Application The quantitative human BSP (glycosylated) sandwich ELISA kit is designed for quantifying glycosylated BSP levels in biological samples. It is particularly useful in studies related to bone metabolism. The kit can be used to assess the effects of drugs on bone formation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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