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Quantitative Human BSP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX302

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Specifications
Product Description
The quantitative human BSP (glycosylated) sandwich ELISA kit is designed to detect human bone sialoprotein (BSP) levels. BSP is a major component of the bone extracellular matrix. It's a highly glycosylated protein that plays a crucial role in bone mineralization. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 55 pg/mL, which can accurately detect low concentrations of BSP in the sample.
Target
BSP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
55 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
150 pg/mL-6000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Bone sialoprotein
Alternate Names
BSP; Bone sialoprotein; IBSP; BspII; Integrin-Binding bone sialoprotein; Bone sialoprotein 2
Uniprot No.
N-Glycosylation Site
104, 177, 182, 190
O-Glycosylation Site
119, 122, 227, 228, 229, 238, 239
Application Notes
Application
The quantitative human BSP (glycosylated) sandwich ELISA kit is designed for quantifying glycosylated BSP levels in biological samples. It is particularly useful in studies related to bone metabolism. The kit can be used to assess the effects of drugs on bone formation.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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