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Quantitative Human CCKAR (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX365

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Specifications
Product Description
The quantitative human CCKAR (glycosylated) sandwich ELISA kit is designed to detect human cholecystokinin A receptor (CCKAR) levels. CCKAR is a G protein-coupled receptor, which binds to cholecystokinin (CCK). CCKAR plays a role in regulating digestion. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 29.852 pg/mL, which can accurately detect low concentrations of CCKAR in the sample.
Target
CCKAR
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
29.852 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
100 pg/mL-4000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Cholecystokinin A receptor
Alternate Names
CCKAR; Cholecystokinin A receptor; CCKRA; CCK1R; Cholecystokinin type 1 receptor
Uniprot No.
N-Glycosylation Site
10, 24, 190
Application Notes
Application
The quantitative human CCKAR (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated CCKAR levels. It is useful for studying the role of CCKAR in pancreatic enzyme secretion.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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