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Quantitative Human CD1A (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX182

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Specifications
Product Description
The quantitative human CD1A (glycosylated) sandwich ELISA kit is designed to detect human T-cell surface glycoprotein CD1A (CD1A ) levels. CD1A is a non-classical MHC class I molecule that presents lipid antigens to T cells, particularly NKT cells, playing a role in immune surveillance and lipid-mediated immune responses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.086 ng/mL, which can accurately detect low concentrations of CD1A in the sample.
Target
CD1A
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.086 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
T-cell surface glycoprotein CD1A
Alternate Names
CD1A ; T-cell surface glycoprotein CD1A; CD1; FCB6; T6; T-cell surface antigen T6/Leu-6
Uniprot No.
N-Glycosylation Site
37, 60, 74, 145
Application Notes
Application
The quantitative human CD1A (glycosylated) sandwich ELISA kit is valuable in research investigating lipid antigen presentation, NKT cell biology, and diseases involving CD1A-expressing cells, such as Langerhans cell histiocytosis and certain T cell lymphomas. Researchers utilize this ELISA to quantify CD1A levels, providing insights into its role in immune responses and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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