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Quantitative Human CD1A (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX182)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human CD1A (glycosylated) sandwich ELISA kit is designed to detect human T-cell surface glycoprotein CD1A (CD1A ) levels. CD1A is a non-classical MHC class I molecule that presents lipid antigens to T cells, particularly NKT cells, playing a role in immune surveillance and lipid-mediated immune responses. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.086 ng/mL, which can accurately detect low concentrations of CD1A in the sample.
Target CD1A
N-Glycosylation Site 37, 60, 74, 145
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.086 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name T-cell surface glycoprotein CD1A
Alternate Names CD1A ; T-cell surface glycoprotein CD1A; CD1; FCB6; T6; T-cell surface antigen T6/Leu-6
Uniprot No. P06126
Application The quantitative human CD1A (glycosylated) sandwich ELISA kit is valuable in research investigating lipid antigen presentation, NKT cell biology, and diseases involving CD1A-expressing cells, such as Langerhans cell histiocytosis and certain T cell lymphomas. Researchers utilize this ELISA to quantify CD1A levels, providing insights into its role in immune responses and potential diagnostic or therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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