Quantitative Human COL4α5 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL4α5 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 4 α5 (COL4α5) levels. COL4α5 is a component of type IV collagen. Type IV collagen is a major component of basement membranes. COL4α5 plays a role in maintaining basement membrane integrity. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 1.253 ng/mL, which can accurately detect low concentrations of COL4α5 in the sample.

COL4α5

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

1.253 ng/mL

Quantitative sandwich ELISA

5 ng/mL-150 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 4 α5

COL4α5; Collagen type 4 α5; COL4-A5; ASLN; Collagen α5(IV) chain

125

The quantitative human COL4α5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated COL4α5 levels. It is useful for studying the role of COL4α5 in basement membrane structure.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 95-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.