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Quantitative Human COL4α5 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX356)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL4α5 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 4 α5 (COL4α5) levels. COL4α5 is a component of type IV collagen. Type IV collagen is a major component of basement membranes. COL4α5 plays a role in maintaining basement membrane integrity. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 1.253 ng/mL, which can accurately detect low concentrations of COL4α5 in the sample.
Target COL4α5
N-Glycosylation Site 125
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 1.253 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 5 ng/mL-150 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 4 α5
Alternate Names COL4α5; Collagen type 4 α5; COL4-A5; ASLN; Collagen α5(IV) chain
Uniprot No. P29400
Application The quantitative human COL4α5 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated COL4α5 levels. It is useful for studying the role of COL4α5 in basement membrane structure.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 95-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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