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Quantitative Human COL5α3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX335)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human COL5α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 5α3 (COL5α3) levels. COL5α3 is a component of type V collagen. Type V collagen is a fibrillar collagen, which is found in the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.071 ng/mL, which can accurately detect low concentrations of COL5α3 in the sample.
Target COL5α3
N-Glycosylation Site 102, 141
O-Glycosylation Site 349
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.071 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Collagen type 5α3
Alternate Names COL5α3; Collagen type 5α3; Collagen α3(V) chain
Uniprot No. P25940
Application The quantitative human COL5α3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated COL5α3 levels. It is useful for studying the role of COL5α3 in collagen fibril formation.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-110%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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