Quantitative Human COL5α3 (Glycosylated) Sandwich ELISA Kit

The quantitative human COL5α3 (glycosylated) sandwich ELISA kit is designed to detect human collagen type 5α3 (COL5α3) levels. COL5α3 is a component of type V collagen. Type V collagen is a fibrillar collagen, which is found in the extracellular matrix. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.071 ng/mL, which can accurately detect low concentrations of COL5α3 in the sample.

COL5α3

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, serum, plasma

100 μL

0.071 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Collagen type 5α3

COL5α3; Collagen type 5α3; Collagen α3(V) chain

102, 141

349

The quantitative human COL5α3 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated COL5α3 levels. It is useful for studying the role of COL5α3 in collagen fibril formation.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-110%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.