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Quantitative Human DSC1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX538

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Specifications
Product Description
The quantitative human DSC1 (glycosylated) sandwich ELISA kit is designed to detect human desmocollin 1 (DSC1) levels. DSC1 is a transmembrane protein that belongs to the cadherin superfamily. Desmocollins are key components of desmosomes, which are cell-cell adhesion structures that provide mechanical strength and stability to tissues. DSC1 is primarily found in stratified squamous epithelia, such as skin. It plays a crucial role in maintaining the integrity of these tissues. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.068 ng/mL, which can accurately detect low concentrations of DSC1 in the sample.
Target
DSC1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.068 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Desmocollin 1
Alternate Names
DSC1; Desmocollin 1; CDHF1; Cadherin family member 1
Uniprot No.
N-Glycosylation Site
165, 546
Application Notes
Application
The quantitative human DSC1 (glycosylated) sandwich ELISA kit is a valuable tool for research in cell biology and dermatology, particularly in studies of cell adhesion and desmosomes in the skin. Researchers use it to measure DSC1 levels in skin tissue samples. The kit has applications in studies of skin diseases and wound healing.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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