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Quantitative Human EAAT4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX433)

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1×48 T
1×96 T

Product Description The quantitative human EAAT4 (glycosylated) sandwich ELISA kit is designed to detect human excitatory amino acid transporter 4 (EAAT4) levels. EAAT4 is a glutamate transporter protein primarily found in the cerebellum, which plays a role in regulating glutamate neurotransmission in this brain region. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.155 ng/mL, which can accurately detect low concentrations of EAAT4 in the sample.
Target EAAT4
N-Glycosylation Site 216, 232, 239
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.155 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Excitatory amino acid transporter 4
Alternate Names EAAT4; Excitatory amino acid transporter 4; SLC1A6
Uniprot No. P48664
Application The quantitative human EAAT4 (glycosylated) sandwich ELISA kit is used to measure glycosylated EAAT4 levels in samples. This measurement is important in neuroscience research, particularly for studying glutamate signaling in the cerebellum. The kit is applied in investigations of conditions affecting cerebellar functions, which helps in understanding the role of EAAT4.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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