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Quantitative Human ECE2 (Glycosylated) Competitive ELISA Kit

CAT#: GLJF-0525-HX215

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Specifications
Product Description
The quantitative human ECE2 (glycosylated) competitive ELISA kit is designed to detect human endothelin converting enzyme 2 (ECE2) levels. ECE2 is a zinc metalloprotease involved in the degradation of endothelin-1 and other bioactive peptides, playing a role in regulating blood pressure and neuronal function. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 50.025 pg/mL, which can accurately detect low concentrations of ECE2 in the sample.
Target
ECE2
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
50.025 pg/mL
Detection Principle
Quantitative competitive ELISA
Detection Range
200 pg/mL-8000 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Endothelin converting enzyme 2
Alternate Names
ECE2; Endothelin converting enzyme 2
Uniprot No.
N-Glycosylation Site
207, 211, 252, 312, 357, 424, 580, 673, 681
Application Notes
Application
The quantitative human ECE2 (glycosylated) competitive ELISA kit is valuable in research investigating cardiovascular diseases, neurological disorders, and renal function. Researchers utilize this kit to quantify ECE2 levels, providing insights into its role in peptide metabolism and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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