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Quantitative Human EPCAM (Glycosylated) Sandwich ELISA Kit, High Sensitivity

CAT#: GLJF-0525-HX260

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Specifications
Product Description
The quantitative human EPCAM (glycosylated) sandwich ELISA kit is designed to detect human epithelial cell adhesion molecule (EPCAM) levels. EPCAM is a protein that is expressed on the surface of epithelial cells. This protein plays a role in cell-cell adhesion, which is also involved in cell signaling and proliferation. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, and plasma. Its sensitivity is 12.528 pg/mL, which can accurately detect low concentrations of EPCAM in the sample.
Target
EPCAM
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates, serum, plasma
Sample Volume
100 μL
Sensitivity
12.528 pg/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
35 pg/mL-1500 pg/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Epithelial cell adhesion molecule
Alternate Names
EPCAM; Epithelial cell adhesion molecule; CD326; TACSTD1; ESA; Epithelial glycoprotein; Tumor associated calcium signal transducer 1
Uniprot No.
N-Glycosylation Site
74, 111, 198
Application Notes
Application
The quantitative human EPCAM (glycosylated) sandwich ELISA kit is designed to measure EPCAM levels in biological samples. Researchers can employ it in studies investigating cancer, particularly carcinomas, and stem cell biology. The kit is a valuable tool in cancer research and the development of new diagnostic methods.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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