Quantitative Human F12 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX131)

Name: Quantitative Human F12 (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX131
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human F12 (glycosylated) sandwich ELISA kit is designed to detect human coagulation factor 12 (F12) levels. F12 is a serine protease that plays a role in the intrinsic pathway of blood coagulation, as well as in the kinin system and fibrinolysis. The kit is suitable for various biological samples such as plasma. Its sensitivity is 0.624 ng/mL, which can accurately detect low concentrations of F12 in the sample.
Target	F12
N-Glycosylation Site	249, 433
O-Glycosylation Site	109, 299, 305, 308, 328, 329, 337
Sample Types	Plasma
Sample Volume	100 μL
Sensitivity	0.624 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	2 ng/mL-100 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Coagulation factor 12
Alternate Names	F12; Coagulation factor 12; FXII; HAF; Hageman factor
Uniprot No.	P00748

Application	The quantitative human F12 (glycosylated) sandwich ELISA kit is valuable in research investigating the role of F12 in thrombosis, inflammation, and related disorders. Researchers utilize this ELISA to quantify F12, aiding in studies concerning coagulation pathways, inflammatory responses, and potential therapeutic interventions.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 80-95%; Plasma sample: n=4, 90-105%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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