Quantitative Human F12 (Glycosylated) Sandwich ELISA Kit

The quantitative human F12 (glycosylated) sandwich ELISA kit is designed to detect human coagulation factor 12 (F12) levels. F12 is a serine protease that plays a role in the intrinsic pathway of blood coagulation, as well as in the kinin system and fibrinolysis. The kit is suitable for various biological samples such as plasma. Its sensitivity is 0.624 ng/mL, which can accurately detect low concentrations of F12 in the sample.

F12

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Plasma

100 μL

0.624 ng/mL

Quantitative sandwich ELISA

2 ng/mL-100 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Coagulation factor 12

F12; Coagulation factor 12; FXII; HAF; Hageman factor

249, 433

109, 299, 305, 308, 328, 329, 337

The quantitative human F12 (glycosylated) sandwich ELISA kit is valuable in research investigating the role of F12 in thrombosis, inflammation, and related disorders. Researchers utilize this ELISA to quantify F12, aiding in studies concerning coagulation pathways, inflammatory responses, and potential therapeutic interventions.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.