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Quantitative Human GPBAR1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX708)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human GPBAR1 (glycosylated) sandwich ELISA kit is designed to detect human g protein-coupled bile acid receptor 1 (GPBAR1) levels. GPBAR1 is a receptor that binds bile acids. GPBAR1 activation triggers signaling pathways. These pathways are involved in metabolic regulation. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.063 ng/mL, which can accurately detect low concentrations of GPBAR1 in the sample.
Target GPBAR1
N-Glycosylation Site 4, 76
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.063 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name G protein-coupled bile acid receptor 1
Alternate Names GPBAR1; G protein-coupled bile acid receptor 1; G-protein coupled receptor GPCR19; hGPCR19
Uniprot No. Q8TDU6
Application The quantitative human GPBAR1 (glycosylated) sandwich ELISA kit is developed for the quantitative analysis of glycosylated GPBAR1 in samples. This measurement is valuable for studies of bile acid signaling. It can be used in investigations of metabolic diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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