Quantitative Human GPBAR1 (Glycosylated) Sandwich ELISA Kit

The quantitative human GPBAR1 (glycosylated) sandwich ELISA kit is designed to detect human g protein-coupled bile acid receptor 1 (GPBAR1) levels. GPBAR1 is a receptor that binds bile acids. GPBAR1 activation triggers signaling pathways. These pathways are involved in metabolic regulation. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.063 ng/mL, which can accurately detect low concentrations of GPBAR1 in the sample.

GPBAR1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Serum, plasma

100 μL

0.063 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

G protein-coupled bile acid receptor 1

GPBAR1; G protein-coupled bile acid receptor 1; G-protein coupled receptor GPCR19; hGPCR19

4, 76

The quantitative human GPBAR1 (glycosylated) sandwich ELISA kit is developed for the quantitative analysis of glycosylated GPBAR1 in samples. This measurement is valuable for studies of bile acid signaling. It can be used in investigations of metabolic diseases.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-100%;
Plasma sample: n=4, 85-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.