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| Size | Qty | Add To Basket |
|---|---|---|
| 1×48 T | ||
| 1×96 T |
| Product Description | The quantitative human GZMA (glycosylated) sandwich ELISA kit is designed to detect human granzyme A (GZMA) levels. GZMA is a serine protease released by cytotoxic T lymphocytes and natural killer cells, playing a crucial role in initiating target cell apoptosis through a caspase-independent pathway. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.062 ng/mL, which can accurately detect low concentrations of GZMA in the sample. |
| Target | GZMA |
| N-Glycosylation Site | 170 |
| Sample Types | Tissue homogenates, cell lysates, serum, plasma |
| Sample Volume | 100 μL |
| Sensitivity | 0.062 ng/mL |
| Detection Principle | Quantitative sandwich ELISA |
| Detection Range | 1 ng/mL-10 ng/mL |
| Detection Time | 1 h-5 h |
| Detection Wavelength | 450 nm |
| Storage | Store at 2-8°C for long term storage. |
| Species | Human |
| Full Name | Granzyme A |
| Alternate Names | GZMA; Granzyme A; CTLA3; HF; Granzyme 1; Hanukkah factor; Cytotoxic T-lymphocyte-associated serine esterase 3 |
| Uniprot No. | P12544 |
| Application | The quantitative human GZMA (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly in cancer immunotherapy, viral infections, and autoimmune diseases. Researchers utilize this ELISA to quantify GZMA levels, providing insights into cytotoxic lymphocyte activity and potential diagnostic or prognostic applications. |
| Kit Components | Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution |
| Precision | Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%; |
| Recovery | Serum sample: n=5, 85-105%; Plasma sample: n=4, 80-100%; |
| Standard Curve | ![]() The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested. |