Quantitative Human GZMA (Glycosylated) Sandwich ELISA Kit

The quantitative human GZMA (glycosylated) sandwich ELISA kit is designed to detect human granzyme A (GZMA) levels. GZMA is a serine protease released by cytotoxic T lymphocytes and natural killer cells, playing a crucial role in initiating target cell apoptosis through a caspase-independent pathway. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.062 ng/mL, which can accurately detect low concentrations of GZMA in the sample.

GZMA

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.062 ng/mL

Quantitative sandwich ELISA

1 ng/mL-10 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Granzyme A

GZMA; Granzyme A; CTLA3; HF; Granzyme 1; Hanukkah factor; Cytotoxic T-lymphocyte-associated serine esterase 3

170

The quantitative human GZMA (glycosylated) sandwich ELISA kit is valuable in research investigating immune responses, particularly in cancer immunotherapy, viral infections, and autoimmune diseases. Researchers utilize this ELISA to quantify GZMA levels, providing insights into cytotoxic lymphocyte activity and potential diagnostic or prognostic applications.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.