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Quantitative Human IgD (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX145

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Specifications
Product Description
The quantitative human IgD (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin D (IgD) levels. IgD is a unique antibody isotype co-expressed with IgM on mature naive B cells, playing a role in B cell activation and differentiation. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 14.825 ng/mL, which can accurately detect low concentrations of IgD in the sample.
Target
IgD
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Serum, plasma
Sample Volume
100 μL
Sensitivity
14.825 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
50 ng/mL-2000 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Immunoglobulin D
Alternate Names
IgD; Immunoglobulin D; IGHD
Uniprot No.
N-Glycosylation Site
225, 316, 367
O-Glycosylation Site
109, 110, 113, 126, 127, 131, 132
Application Notes
Application
The quantitative human IgD (glycosylated) sandwich ELISA kit is utilized in research to explore the role of IgD in immune regulation, B cell development, and certain disease states. Researchers use this ELISA to quantify IgD levels, providing insights into its involvement in immune responses and potential clinical implications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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