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Quantitative Human IgD (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX145)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IgD (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin D (IgD) levels. IgD is a unique antibody isotype co-expressed with IgM on mature naive B cells, playing a role in B cell activation and differentiation. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 14.825 ng/mL, which can accurately detect low concentrations of IgD in the sample.
Target IgD
N-Glycosylation Site 225, 316, 367
O-Glycosylation Site 109, 110, 113, 126, 127, 131, 132
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 14.825 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 50 ng/mL-2000 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin D
Alternate Names IgD; Immunoglobulin D; IGHD
Uniprot No. P01880
Application The quantitative human IgD (glycosylated) sandwich ELISA kit is utilized in research to explore the role of IgD in immune regulation, B cell development, and certain disease states. Researchers use this ELISA to quantify IgD levels, providing insights into its involvement in immune responses and potential clinical implications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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