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Quantitative Human IgG2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX141)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IgG2 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G2 (IgG2) levels. IgG2 is an IgG subclass involved in humoral immunity, particularly against polysaccharide antigens. It plays a role in defense against encapsulated bacteria and contributes to overall antibody responses. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.723 µg/mL, which can accurately detect low concentrations of IgG2 in the sample.
Target IgG2
N-Glycosylation Site 176
Sample Types Serum, plasma
Sample Volume 100 μL
Sensitivity 0.723 µg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 5 µg/mL-100 µg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin G2
Alternate Names IgG2; Immunoglobulin G2; IGHG2; Immunoglobulin heavy constant gamma 2
Uniprot No. P01859
Application The quantitative human IgG2 (glycosylated) sandwich ELISA kit is useful in research and clinical settings to assess IgG2 levels, particularly in studies concerning immune responses to polysaccharide vaccines and in conditions involving bacterial infections. Researchers and clinicians employ this ELISA to quantify IgG2, aiding in the evaluation of immune function and vaccine efficacy.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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