Quantitative Human IgG2 (Glycosylated) Sandwich ELISA Kit

The quantitative human IgG2 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G2 (IgG2) levels. IgG2 is an IgG subclass involved in humoral immunity, particularly against polysaccharide antigens. It plays a role in defense against encapsulated bacteria and contributes to overall antibody responses. The kit is suitable for various biological samples such as serum, plasma. Its sensitivity is 0.723 µg/mL, which can accurately detect low concentrations of IgG2 in the sample.

IgG2

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Serum, plasma

100 μL

0.723 µg/mL

Quantitative sandwich ELISA

5 µg/mL-100 µg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Immunoglobulin G2

IgG2; Immunoglobulin G2; IGHG2; Immunoglobulin heavy constant gamma 2

176

The quantitative human IgG2 (glycosylated) sandwich ELISA kit is useful in research and clinical settings to assess IgG2 levels, particularly in studies concerning immune responses to polysaccharide vaccines and in conditions involving bacterial infections. Researchers and clinicians employ this ELISA to quantify IgG2, aiding in the evaluation of immune function and vaccine efficacy.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-100%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.