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Quantitative Human IgG4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX142)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human IgG4 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G4 (IgG4) levels. IgG4 is an IgG subclass involved in chronic inflammatory responses and immune tolerance, often associated with prolonged antigen exposure. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.120 µg/mL, which can accurately detect low concentrations of IgG4 in the sample.
Target IgG4
N-Glycosylation Site 177
Sample Types Tissue homogenates, cell lysates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.120 µg/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 µg/mL-10 µg/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Immunoglobulin G4
Alternate Names IgG4; Immunoglobulin G4; IGHG4; Immunoglobulin heavy constant gamma 4
Uniprot No. P01861
Application The quantitative human IgG4 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for investigating IgG4-related diseases, allergic reactions, and immune tolerance. Researchers and clinicians use this ELISA to quantify IgG4 levels, providing insights into disease pathogenesis and aiding in diagnosis and monitoring.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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