Quantitative Human IgG4 (Glycosylated) Sandwich ELISA Kit

The quantitative human IgG4 (glycosylated) sandwich ELISA kit is designed to detect human immunoglobulin G4 (IgG4) levels. IgG4 is an IgG subclass involved in chronic inflammatory responses and immune tolerance, often associated with prolonged antigen exposure. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, serum, plasma. Its sensitivity is 0.120 µg/mL, which can accurately detect low concentrations of IgG4 in the sample.

IgG4

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates, serum, plasma

100 μL

0.120 µg/mL

Quantitative sandwich ELISA

1 µg/mL-10 µg/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Immunoglobulin G4

IgG4; Immunoglobulin G4; IGHG4; Immunoglobulin heavy constant gamma 4

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The quantitative human IgG4 (glycosylated) sandwich ELISA kit is valuable in research and clinical settings for investigating IgG4-related diseases, allergic reactions, and immune tolerance. Researchers and clinicians use this ELISA to quantify IgG4 levels, providing insights into disease pathogenesis and aiding in diagnosis and monitoring.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 80-95%;
Plasma sample: n=4, 90-105%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.