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Quantitative Human IL18RAP (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX110

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Specifications
Product Description
The quantitative human IL18RAP (glycosylated) sandwich ELISA kit is designed to detect human interleukin 18 receptor accessory protein (IL18RAP) levels. IL18RAP is a co-receptor that forms a functional signaling complex with the IL-18 receptor, crucial for IL-18-mediated immune responses. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.073 ng/mL, which can accurately detect low concentrations of IL18RAP in the sample.
Target
IL18RAP
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.073 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Interleukin 18 receptor accessory protein
Alternate Names
IL18RAP; Interleukin 18 receptor accessory protein; CDw218b; CD218 Antigen-like family member B
Uniprot No.
N-Glycosylation Site
21, 119, 152, 345
Application Notes
Application
The quantitative human IL18RAP (glycosylated) sandwich ELISA kit is valuable for research investigating inflammatory diseases, autoimmune disorders, and cancer, where IL-18 signaling plays a significant role. The quantitative data obtained from this kit allows for precise analysis of IL18RAP levels, contributing to a better understanding of its involvement in these pathological processes and potential therapeutic interventions.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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