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Quantitative Human LEFTY1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX92)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human LEFTY1 (glycosylated) sandwich ELISA kit is designed to detect human left determination factor 1 (LEFTY1) levels. LEFTY1 is a secreted protein that acts as an antagonist of Nodal signaling, playing a crucial role in left-right axis determination during embryonic development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.592 ng/mL, which can accurately detect low concentrations of LEFTY1 in the sample.
Target LEFTY1
N-Glycosylation Site 158
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.592 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Left determination factor 1
Alternate Names LEFTY1; Left determination factor 1; LEFTYB; LEFTB; Left right determination factor B
Uniprot No. O75610
Application The quantitative human LEFTY1 (glycosylated) sandwich ELISA kit is valuable for research investigating developmental disorders, particularly those related to left-right asymmetry, and cancer biology. The quantitative data obtained from this kit allows for precise analysis of LEFTY1 levels, contributing to a better understanding of its role in embryogenesis and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 95-110%;
Plasma sample: n=4, 85-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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