Quantitative Human LEFTY1 (Glycosylated) Sandwich ELISA Kit

The quantitative human LEFTY1 (glycosylated) sandwich ELISA kit is designed to detect human left determination factor 1 (LEFTY1) levels. LEFTY1 is a secreted protein that acts as an antagonist of Nodal signaling, playing a crucial role in left-right axis determination during embryonic development. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.592 ng/mL, which can accurately detect low concentrations of LEFTY1 in the sample.

LEFTY1

Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution

Tissue homogenates, cell lysates

100 μL

0.592 ng/mL

Quantitative sandwich ELISA

2 ng/mL-40 ng/mL

1 h-5 h

450 nm

Store at 2-8°C for long term storage.

Human

Left determination factor 1

LEFTY1; Left determination factor 1; LEFTYB; LEFTB; Left right determination factor B

158

The quantitative human LEFTY1 (glycosylated) sandwich ELISA kit is valuable for research investigating developmental disorders, particularly those related to left-right asymmetry, and cancer biology. The quantitative data obtained from this kit allows for precise analysis of LEFTY1 levels, contributing to a better understanding of its role in embryogenesis and disease pathogenesis.

Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;

Serum sample: n=5, 95-110%;
Plasma sample: n=4, 85-95%;

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.