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Quantitative Human MHCE (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX240

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Specifications
Product Description
The quantitative human MHCE (glycosylated) sandwich ELISA kit is designed to detect human major histocompatibility complex class 1E (MHCE) levels. MHCE is a non-classical MHC class I molecule involved in antigen presentation and immune modulation, particularly in non-conventional T cell responses. It may play a role in regulating immune tolerance and responses. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.077 ng/mL, which can accurately detect low concentrations of MHCE in the sample.
Target
MHCE
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.077 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Major histocompatibility complex class 1E
Alternate Names
MHCE; Major histocompatibility complex class 1E; HLAE; Leukocyte antigen E; MHC class I antigen E
Uniprot No.
N-Glycosylation Site
107
Application Notes
Application
The quantitative human MHCE (glycosylated) sandwich ELISA kit is valuable in research investigating immune regulation, transplantation immunology, and autoimmune diseases, where MHCE expression and function are relevant. Researchers utilize this ELISA to quantify MHCE levels, providing insights into its role in immune responses and potential diagnostic or therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-105%;
Plasma sample: n=4, 95-110%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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