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Quantitative Human PAR2 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX474)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PAR2 (glycosylated) sandwich ELISA kit is designed to detect human protease activated receptor 2 (PAR2) levels. PAR2 is a G protein-coupled receptor that is activated by certain proteases. PAR2 activation is involved in various cellular responses, which plays a role in inflammation and pain signaling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.100 ng/mL, which can accurately detect low concentrations of PAR2 in the sample.
Target PAR2
N-Glycosylation Site 30, 222
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.100 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protease activated receptor 2
Alternate Names PAR2; Protease activated receptor 2; F2RL1; GPR11; G-protein coupled receptor 11; Thrombin receptor-like 1
Uniprot No. P55085
Application The quantitative human PAR2 (glycosylated) sandwich ELISA kit is designed to measure glycosylated PAR2 levels in samples. This measurement is valuable in research related to inflammation. The kit is applied in studies of pain signaling, which helps in understanding the role of PAR2.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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