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Quantitative Human SCUBE3 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX681

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Specifications
Product Description
The quantitative human SCUBE3 (glycosylated) sandwich ELISA kit is designed to detect human signal peptide, CUB and EGF-like domain-containing protein 3 (SCUBE3) levels. SCUBE3 is secreted, which plays a role in cell signaling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of SCUBE3 in the sample.
Target
SCUBE3
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.087 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Signal peptide, CUB and EGF-like domain-containing protein 3
Alternate Names
SCUBE3; Signal peptide, CUB and EGF-like domain-containing protein 3
Uniprot No.
N-Glycosylation Site
417, 464, 685, 756, 785
Application Notes
Application
The quantitative human SCUBE3 (glycosylated) sandwich ELISA kit is used to determine SCUBE3 levels in biological specimens. This measurement is crucial for studies investigating cell signaling pathways. It also contributes to understanding the role of SCUBE3 in development. Furthermore, it is valuable in research exploring embryogenesis and related processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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