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Quantitative Human SCUBE3 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX681)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SCUBE3 (glycosylated) sandwich ELISA kit is designed to detect human signal peptide, CUB and EGF-like domain-containing protein 3 (SCUBE3) levels. SCUBE3 is secreted, which plays a role in cell signaling. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.087 ng/mL, which can accurately detect low concentrations of SCUBE3 in the sample.
Target SCUBE3
N-Glycosylation Site 417, 464, 685, 756, 785
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.087 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Signal peptide, CUB and EGF-like domain-containing protein 3
Alternate Names SCUBE3; Signal peptide, CUB and EGF-like domain-containing protein 3
Uniprot No. Q8IX30
Application The quantitative human SCUBE3 (glycosylated) sandwich ELISA kit is used to determine SCUBE3 levels in biological specimens. This measurement is crucial for studies investigating cell signaling pathways. It also contributes to understanding the role of SCUBE3 in development. Furthermore, it is valuable in research exploring embryogenesis and related processes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 90-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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