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Quantitative Human SGLT1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX241

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Specifications
Product Description
The quantitative human SGLT1 (glycosylated) sandwich ELISA kit is designed to detect human sodium-glucose cotransporter 1 (SGLT1) levels. SGLT1 is a membrane protein that mediates the active transport of glucose and sodium across cell membranes, primarily in the intestine and kidneys. It plays a crucial role in glucose absorption and reabsorption. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of SGLT1 in the sample.
Target
SGLT1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates
Sample Volume
100 μL
Sensitivity
0.083 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sodium-glucose cotransporter 1
Alternate Names
SGLT1; Sodium-glucose cotransporter 1; SLC5A1; Solute carrier family 5 member 1
Uniprot No.
N-Glycosylation Site
248
Application Notes
Application
The quantitative human SGLT1 (glycosylated) sandwich ELISA kit is valuable in research investigating diabetes, intestinal glucose absorption, and renal glucose handling. Researchers utilize this ELISA to quantify SGLT1 levels, providing insights into its role in glucose homeostasis and potential therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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