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Quantitative Human SGLT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX241)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SGLT1 (glycosylated) sandwich ELISA kit is designed to detect human sodium-glucose cotransporter 1 (SGLT1) levels. SGLT1 is a membrane protein that mediates the active transport of glucose and sodium across cell membranes, primarily in the intestine and kidneys. It plays a crucial role in glucose absorption and reabsorption. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of SGLT1 in the sample.
Target SGLT1
N-Glycosylation Site 248
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.083 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Sodium-glucose cotransporter 1
Alternate Names SGLT1; Sodium-glucose cotransporter 1; SLC5A1; Solute carrier family 5 member 1
Uniprot No. P13866
Application The quantitative human SGLT1 (glycosylated) sandwich ELISA kit is valuable in research investigating diabetes, intestinal glucose absorption, and renal glucose handling. Researchers utilize this ELISA to quantify SGLT1 levels, providing insights into its role in glucose homeostasis and potential therapeutic applications.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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