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Quantitative Human SIRP-β1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX11

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Specifications
Product Description
The quantitative human SIRP-β1 (glycosylated) sandwich ELISA kit is designed to detect human signal regulatory protein β1 (SIRP-β1) levels. SIRPβ1 is a transmembrane protein involved in cell adhesion and signaling, particularly in myeloid cells, and it contributes to immune responses and cellular interactions. It plays a role in regulating cell-cell interactions and signal transduction. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.132 ng/mL, which can accurately detect low concentrations of SIRP-β1 in the sample.
Target
SIRP-β1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, cell lysates
Sample Volume
100 μL
Sensitivity
0.132 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.55 ng/mL-15 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Signal regulatory protein β1
Alternate Names
SIRP-β1; Signal regulatory protein β1; CD172b; SIRP-B1; CD172 antigen-like family member B
Uniprot No.
N-Glycosylation Site
244, 269, 291
Application Notes
Application
The quantitative human SIRP-β1 (glycosylated) sandwich ELISA kit is useful for researchers studying immune regulation, cancer biology, and inflammatory diseases, where SIRP-β1 plays a role in cellular communication. It enables the quantification of SIRP-β1 levels, aiding in the investigation of its involvement in disease pathogenesis and potential therapeutic applications.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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