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Quantitative Human Slit1 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX84

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Specifications
Product Description
The quantitative human Slit1 (glycosylated) sandwich ELISA kit is designed to detect human slit homolog 1 (Slit1) levels. Slit1 is a secreted protein that plays a crucial role in axonal guidance, cell migration, and angiogenesis through its interaction with Robo receptors. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.096 ng/mL, which can accurately detect low concentrations of Slit1 in the sample.
Target
Slit1
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.096 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Slit homolog 1
Alternate Names
Slit1; Slit homolog 1; MEGF4; SLIL1; SLIT3; Multiple epidermal growth factor-like domains protein 4
Uniprot No.
N-Glycosylation Site
72, 192, 406, 571, 630, 762, 801, 806, 1026, 1079, 1189, 1259, 1306
Application Notes
Application
The quantitative human slit1 (glycosylated) sandwich ELISA kit is valuable for research investigating neuronal development, cancer metastasis, and vascular biology. The quantitative data obtained from this kit allows for precise analysis of slit1 levels, contributing to a better understanding of its role in these complex biological processes.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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