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Quantitative Human Slit1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX84)

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1×48 T
1×96 T

Product Description The quantitative human Slit1 (glycosylated) sandwich ELISA kit is designed to detect human slit homolog 1 (Slit1) levels. Slit1 is a secreted protein that plays a crucial role in axonal guidance, cell migration, and angiogenesis through its interaction with Robo receptors. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.096 ng/mL, which can accurately detect low concentrations of Slit1 in the sample.
Target Slit1
N-Glycosylation Site 72, 192, 406, 571, 630, 762, 801, 806, 1026, 1079, 1189, 1259, 1306
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.096 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Slit homolog 1
Alternate Names Slit1; Slit homolog 1; MEGF4; SLIL1; SLIT3; Multiple epidermal growth factor-like domains protein 4
Uniprot No. O75093
Application The quantitative human slit1 (glycosylated) sandwich ELISA kit is valuable for research investigating neuronal development, cancer metastasis, and vascular biology. The quantitative data obtained from this kit allows for precise analysis of slit1 levels, contributing to a better understanding of its role in these complex biological processes.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-100%;
Plasma sample: n=4, 80-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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