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Quantitative Human WNT5B (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0325-HX1230

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Specifications
Product Description
The quantitative human WNT5B (glycosylated) sandwich ELISA kit is designed to detect human wingless-type MMTV integration site family, member 5B (WNT5B) levels. It is suitable for various biological samples such as tissue homogenates, cell culture supernates, serum, or plasma. Its sensitivity is 0.042 ng/mL, which can accurately detect low concentrations of WNT5B in the sample.
Target
WNT5B
Sample Types
Tissue homogenates, cell culture supernates, serum, plasma
Sample Volume
50 μL-100 μL
Sensitivity
0.042 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
0.16 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Wingless-type MMTV integration site family, member 5B
Alternate Names
Wingless-type MMTV integration site family, member 5B; Protein Wnt-5b; WNT 5B protein; Wnt family member 5B; WNT5B
Uniprot No.
N-Glycosylation Site
93, 99, 291, 305
Application Notes
Application
The quantitative human WNT5B (glycosylated) sandwich ELISA kit is widely used to assess the expression levels of WNT5B, helping to understand its role in stem cells.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 90-105%;
Plasma sample: n=4, 80-90%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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