Recombinant Northern cereal mosaic virus (NCMV) Glycoprotein (G) (22-483 aa), E. coli

Recombinant Northern cereal mosaic virus (NCMV) Glycoprotein (22-483 aa) was expressed in E. coli with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Northern cereal mosaic virus (NCMV)

22-483 aa

LSILSCNKTDEVPTVTQCFKSCSDTILGEQVKVSILSQEDPTSIVVGRCIWRIMKQSFTE TWTFSRLISAKEITWEPATTQECNGAFHNLCKNKAGCVSEDLEIEPEFSWARTEIREVKH LSIETLTMSAYLHQGAGKVLIDGVAVPISKKTHNNGDFTYVWDDVPISEVCPWKHPISHL SCYKDKEDLDDIYCPSQGISLVNYTKVDTSCPEQIYTDVGGLVFKLGKVDFNDWYPYVIE SSDVAVKETISSINMALKMRESVHCHQDCLEIRRRVTYVDGFYYDPLPPAKCRLIGNCSV DSGSVTCNNGTLVWATCGGRRVWIDLKSGRDVKNAVCEKGGRSRISKNQFEGVLNEFHLN NSKFGNILRANEIHSVILKDNEVMDFARVVKSASERSGNFSEGTLINVRQLFRPMINFLR GIEHEIKVVAFSVLALIVGYIIIRIRTVTVAKAKSLESIAMI

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

G

Glycoprotein

Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane.

Glycoprotein