Recombinant Sudan ebolavirus Pre-small/secreted glycoprotein (GP) (33-372 aa), Baculovirus

Recombinant Sudan ebolavirus (strain Boniface-76) (SEBOV) Pre-small/secreted glycoprotein (33-372 aa) was expressed in Baculovirus with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Baculovirus

Sudan ebolavirus (strain Boniface-76) (SEBOV)

33-372 aa

MPLGVVTN STLEVTEIDQ LVCKDHLAST DQLKSVGLNL EGSGVSTDIP SATKRWGFRS GVPPQVVSYE AGEWAENCYN LEIKKPDGSE CLPPPPDGVR GFPRCRYVHK AQGTGPCPGD YAFHKDGAFF LYDRLASTVI YRGVNFAEGV IAFLILAKPK ETFLQSPPIR EAANYTENTS SYYATSYLEY EIENFGAQHS TTLFKINNNT FVLLDRPHTP QFLFQLNDTI QLHQQLSNTT GKLIWTLDAN INADIGEWAF WENKKISPNN YVEKSCLSKL YRSTRQKTMM RHRRELQREE SPTGPPGSIR TWFQRIPLGW FHCTYQKGKQ HCRLRIRQKV EE

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

GP

Pre-small/secreted glycoprotein

sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils.

GP; Pre-small/secreted glycoprotein; pre-sGP; Small/secreted glycoprotein; sGP; Delta-peptide