Recombinant Zaire ebolavirus (strain Mayinga-76) Pre-small/secreted glycoprotein (GP) (33-364 aa), E. coli

Recombinant Zaire ebolavirus (strain Mayinga-76) (ZEBOV) Pre-small/secreted glycoprotein (NP_066247.1) (33-364 aa) was expressed in E. coli with a His-tag or Tag free. The biological activity was determined by its binding ability in a functional ELISA. This product can be used in ELISA, WB, and IP.

Determined by its binding ability in a functional ELISA.

Zaire ebolavirus (strain Mayinga-76) (ZEBOV)

33-364 aa

IPLGVIHN STLQVSDVDK LVCRDKLSST NQLRSVGLNL EGNGVATDVP SATKRWGFRS GVPPKVVNYE AGEWAENCYN LEIKKPDGSE CLPAAPDGIR GFPRCRYVHK VSGTGPCAGD FAFHKEGAFF LYDRLASTVI YRGTTFAEGV VAFLILPQAK KDFFSSHPLR EPVNATEDPS SGYYSTTIRY QATGFGTNET EYLFEVDNLT YVQLESRFTP QFLLQLNETI YTSGKRSNTT GKLIWKVNPE IDTTIGEWAF WETKKTSLEK FAVKSCLSQL YQTEPKTSVV RVRRELLPTQ GPTQQLKTTK SWLQKIPLQW FKCTVKEGKL QCRI

His-tag or Tag free

>90%, determined by SDS-PAGE.

Unconjugated

GP

Pre-small/secreted glycoprotein

sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha. Might therefore contribute to the lack of inflammatory reaction seen during infection in spite the of extensive necrosis and massive virus production. Does not seem to be involved in activation of primary macrophages. Does not seem to interact specifically with neutrophils.

GP; Pre-small/secreted glycoprotein; pre-sGP; Small/secreted glycoprotein; sGP; Delta-peptide