CE is the key Glycan Tool Enzyme for lactose-to-lactulose conversion, while CE revolutionizes sustainable bioconversion of lignocellulosic biomass by transforming agricultural waste into high-value products. Our custom CE production services exploit its unique reversible epimerization activity to convert cellobiose into xylobiose or rare sugars, bridging low-cost feedstocks with premium bio-based chemicals such as prebiotics, pharmaceutical intermediates, and biodegradable polymers. Unlike wild-type variants, our engineered CE enzymes offer unparalleled regioselectivity and industrial-scale stability, ensuring consistent performance in large-scale biorefineries. By partnering with us, clients gain access to tailored CE solutions that accelerate circular economy transitions, reduce reliance on fossil fuels, and meet stringent sustainability targets across food, energy, and advanced materials sectors.

Fig. 1. MEF-induced C2E conformational changes and catalytic effects. (OA Literature) Fig.1 Proposed conceptual model of MEFs-induced conformational changes in C2E and their catalytic implications.¹

Custom CE Production Workflow

Step 1

In Silico Enzyme Modeling

We employ cutting-edge molecular dynamics (MD) simulations as a powerful tool to identify flexible loops within the Epimerase structure. Additionally, these simulations enable us to pinpoint potential sites that are suitable for rigidification through targeted mutations.

Step 2

Gene Synthesis & Expression Vector Construction

Once we have optimized the enzyme sequences through in silico modeling, we proceed to synthesize these optimized sequences with high precision. Subsequently, we clone these synthesized sequences into high-efficiency expression hosts.

Step 3

High-Throughput Screening

To evaluate the performance of the enzyme variants, we employ high-throughput screening techniques. Using automated micro-bioreactors, we screen these variants for their thermostability, which is a key factor in determining their suitability for industrial applications.

Step 4

Process Intensification & Scale-up

After identifying the top-performing enzyme with high-throughput screening, we transition it to a larger-scale fermentation process. By conducting fermentation on a larger scale, we can better understand how the enzyme performs under real-world industrial conditions and make any necessary adjustments to optimize its production.

Step 5

Quality Control & Characterization

Before the final enzymes are ready for commercial use, they undergo rigorous quality control and characterization procedures. Through comprehensive quality control and characterization steps, we ensure that the final enzymes meet the highest standards of quality and performance.

Fig. 2. Custom CE production workflow. (Creative Biolabs AI) Fig.2 Workflow of CE production.

Discover How We Accelerate Research Breakthroughs!

Service Highlights

Tailored Performance
Our platform delivers custom CE variants optimized for stability, specificity, and efficiency, enabling breakthroughs across industries. With gene-editing precision, we engineer CE enzymes that outperform wild-type counterparts.

Flexible Host Systems
Choose the ideal expression system to match your budget and timeline. E. coli offers rapid, low-cost scaling, ideal for R&D and early-stage production. For harsh industrial conditions, Pichia pastoris adds glycosylation to enhance stability under extreme pH or temperature. Need pharmaceutical-grade modifications? Insect cells enable complex post-translational modifications, such as disulfide bonds or phosphorylation, ensuring compliance with stringent regulatory standards. This flexibility cuts development timelines compared to fixed-host providers.

Seamless Scalability
Our process guarantees >95% purity and >90% activity retention at every scale, eliminating the scale-up penalty common in enzyme production. Whether you need 10 mg R&D samples or 10+ kg industrial batches, we ensure consistency, even in multi-ton campaigns.

Uncompromising Quality
Every batch undergoes rigorous triple-validated testing to ensure functional integrity: purity is confirmed through SDS-PAGE, HPLC, and MALDI-TOF MS; activity is validated via cell-free assays with cellobiose/NADH coupling, achieving HPLC-verified conversion rates exceeding 95%; and structural accuracy is analyzed at the atomic level using X-ray crystallography or NMR, guaranteeing consistent performance in complex biological systems.

Applications

Biomass Breakdown for Biofuels

By epimerizing cellobiose into epilactose, CE accelerates lignocellulosic biomass degradation, lowering energy/chemical demands in biofuel production. This supports the sustainable conversion of agricultural waste into renewable energy sources like bioethanol.

Food Texture & Stability Enhancement

CE modifies lactose into epilactose, improving dairy creaminess and reducing lactose content for intolerant consumers. Its rare sugar derivatives also act as natural sweeteners/humectants, extending shelf life and enabling clean-label formulations.

Prebiotic Gut Health Support

CE-generated tagatose and epilactose selectively boost beneficial gut bacteria, producing short-chain fatty acids that enhance immunity and metabolism. This drives innovation in prebiotic-rich functional foods and nutraceuticals.

Industrial Enzyme Optimization

Engineered CE variants with improved stability and substrate specificity enable efficient biocatalysis in harsh conditions (e.g., high temperatures, extreme pH). This expands its use in biorefineries, pharmaceutical synthesis, and eco-friendly chemical manufacturing.

Partnering with Us to Achieve Your Scientific Goals!

Customer Reviews

High Stability
"Using custom CE in our research has significantly facilitated our high-temperature isomerization trials. The R17Q variant maintained activity at 75°C far longer than the wild-type we previously used, allowing for much cleaner batches."- 14 months ago, Prof. M***e L.

Significant Effect
"The reduction in glucose-byproduct formation using engineered epimerase in our prebiotic research area has been impressive. It simplified our HPLC purification steps immensely compared to traditional alkaline methods."- 6 months ago, Prof. M***e S.

FAQs

Q: How does your custom CE production service differ from off-the-shelf enzymes?

A: Unlike generic enzymes, our service provides tailor-engineered CE variants optimized for client-specific needs, including thermostability, substrate specificity, or catalytic efficiency. We also offer flexible host systems (E. coli, insect cells) to match scale, cost, and post-translational modification requirements.

Q: Can you engineer CE for extreme industrial conditions?

A: Absolutely. Our gene-editing platform designs CE variants with industrial-grade stability.

Q: How do you ensure batch consistency and scalability for industrial CE production?

A: Our process guarantees >95% purity and >90% activity retention across scales, from 10 mg R&D samples to 10+ kg industrial batches.

Q: What quality control measures are in place for custom CE?

A: Every batch undergoes triple-validated testing:

Purity: Confirmed via SDS-PAGE, HPLC, and MALDI-TOF MS.

Activity: Validated through cell-free assays.

Structure: Analyzed by X-ray crystallography/NMR for atomic-level accuracy.

Extended Services

Custom Lactulose Production Service

Tailored to your specific needs, our custom lactulose production service delivers high-quality lactulose solutions with precision and efficiency, ensuring optimal results for your applications.

Carbohydrate Analysis Services

Our carbohydrate analysis services provide comprehensive, accurate, and timely assessments of carbohydrate profiles, empowering you with critical data.

How to Contact Us?

Ready to revolutionize your bioconversion workflow? Our CE enzyme solutions are engineered to unlock the full potential of cellulosic waste, turning low-value biomass into high-value products like rare sugars, biofuels, and prebiotics. Contact our experts today to design a tailored strategy that accelerates your sustainability goals while boosting efficiency and profitability.

Reference

Albuquerque, Tiago Lima de, et al. "Modulation of Enzymatic Activity by Moderate Electric Fields: Perspectives for Prebiotic Epilactose Production via Cellobiose-2-Epimerase." Processes 13.9 (2025): 2671. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/pr13092671.

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