DTEase, a member of the ketose 3-epimerase family, serves as a critical Glycosyl Functional Enzyme in the stereospecific interconversion of rare sugars. This Epimerase exhibits broad substrate specificity, catalyzing the reversible epimerization of ketoses at the C-3 position through a deprotonation-reprotonation mechanism mediated by a conserved histidine residue. Specifically, DTEase enables the efficient interconversion between D-tagatose and D-sorbose, as well as between D-fructose and D-allulose.

Fig. 1. Key physiological impacts of D-tagatose in humans. (OA Literature) Fig.1 Various physiological functions of D-tagatose in the human body.¹

Service Overview

Are you currently facing low conversion rates, thermal instability of enzymes, or cost-intensive purification cycles in your rare sugar manufacturing? Our custom DTEase production service helps you obtain high-activity, thermotolerant enzymes and achieve up to 85% conversion rates through our AI-augmented rational design and certified Bacillus subtilis expression platforms. We streamline your path from molecular design to industrial-scale D-allulose and D-tagatose production.

Discover How We Support High-Impact Discoveries

DTEase Production Workflow

Step 1

Al-Guided Enzyme Redesign

We leverage advanced AI-guided design methods to precisely identify structural "hotspots" in the DTE scaffold. These hotspots are targeted to significantly boost the enzyme's thermotolerance, allowing it to function effectively at higher temperatures, and enhance its substrate affinity for improved binding and conversion efficiency. This focused approach ensures superior enzyme performance for industrial use.

Step 2

Expression Host Engineering

The optimized gene sequence, crafted through extensive genetic engineering and modeling, is synthesized with precision and integrated into high-secretion vectors. These vectors are designed to facilitate efficient extracellular secretion of the enzyme, simplifying downstream processing by reducing the need for complex purification and enhancing overall yield and quality.

Step 3

Fermentation Optimization

Through controlled fed-batch fermentation experiments, we explore the impact of metal ion concentrations and pH buffers on enzymatic activity. By systematically varying these conditions and analyzing the data, we determine the optimal combination that maximizes enzyme activity, ensuring consistent and high-quality production.

Step 4

Enzyme Immobilization and Stability Assessment

To ensure long-term durability and reusability, the DTE enzyme is immobilized on advanced resin supports with high surface area and chemical stability. Rigorous stability testing evaluates the enzyme's half-life and recycling stability over multiple 24-hour cycles, verifying its ability to maintain activity and structural integrity under repeated use for reliable and cost-effective industrial applications.

Step 5

Pilot-Scale Validation

A 10-liter scale run validates the enzyme's performance in a realistic industrial setting, bridging the gap between lab research and full-scale production. We aim for a conversion rate exceeding 85%, demonstrating high efficiency, and closely monitor product purity to meet stringent quality standards. Successful validation confirms the enzyme's reliability, paving the way for final delivery and commercialization.

Fig. 2. DTEase production workflow. (Creative Biolabs AI) Fig.2 DTEase production procedure.

Why Choose Us?

Proprietary AI Optimization
Our proprietary AI optimization platform utilizes machine learning to predict mutations that substantially boost the melting temperature and catalytic turnover of enzymes. By analyzing vast enzyme datasets, we pinpoint mutations that ensure enzymes maintain stability and activity under extreme conditions while accelerating substrate processing.

Industrial-Grade Stability
Stability is key in industry, and our enzymes are designed for it. They can withstand high substrate concentrations without inhibition, ensuring consistent catalytic activity. This resilience maximizes reactor throughput, allowing for higher production volumes. Plus, it reduces the need for frequent enzyme additions, enhancing operational efficiency and minimizing downtime, even in challenging environments.

End-to-End Scalability
Scaling up enzyme technology is vital for your success. We offer more than just an enzyme; we provide a complete, validated scale-up protocol based on real-world pilot data. Our protocol covers reactor design, process optimization, and quality control, ensuring a seamless transition from lab experiments to large-scale production.

Reduced Operational Expenses
Our enzymes deliver high-yield conversion rates, minimizing substrate waste and maximizing product yield, which lowers raw material costs. Their superior immobilization stability keeps them firmly attached, reducing enzyme replacement frequency and maintenance costs. Additionally, they simplify downstream purification, further cutting costs.

Collaborating to Amplify Your Scientific Impact!

Key Applications

Low-Calorie Sweetener Production

Efficiently converting bulk fructose into D-allulose, providing a 70% sweetness profile with nearly zero calories.

Pharmaceutical Intermediates

Synthesizing rare sugar backbones used in the development of antiviral agents and specific glycoproteins.

Functional Food Ingredients

Producing D-tagatose for use in low-glycemic index (GI) products and prebiotic formulations.

Specialty Nutraceuticals

Enabling the cost-effective synthesis of rare hexoses that exhibit unique anti-inflammatory or antioxidant properties.

Precision Fermentation

Serving as a primary catalytic component in engineered microbial consortia for complex carbon source utilization.

Customer Reviews

DTE Facilitation
"Using the custom DTE production service in our research has significantly facilitated our high-density substrate trials. The enzyme's stability at 70°C allowed us to run continuous batches without the usual loss of activity seen with wild-type strains."- 2 weeks ago, Dr. Al*rt M.

Low Endotoxin
"The transition to the certified expression host improved our downstream processing speed by 40%. The lack of endotoxins made our D-tagatose application much safer for food-grade testing."- 6 weeks ago, Prof. Sa*ah L.

FAQs

Q: What is the primary advantage of using your custom enzyme over commercial off-the-shelf DTE?

A: Off-the-shelf enzymes often lack the thermal stability needed for industrial runs. Our custom service uses AI to "harden" the enzyme for your specific reactor conditions, ensuring longer half-lives and higher cumulative yields.

Q: Do you offer enzyme immobilization as part of the service?

A: Absolutely. We offer several immobilization options (adsorption, covalent bonding, etc.) to ensure the enzyme can be reused across multiple batches, significantly lowering your COGS.

Q: How do you ensure the purity of the D-allulose or D-tagatose produced?

A: Our engineered DTE variants are designed for high specificity. We provide HPLC data with every batch to verify that side-product formation (like D-glucose or D-fructose residues) is within your required tolerances.

Q: What if my project requires a different pH than the standard 8.0?

A: Our rational design process includes "pH-shifting" engineering. Please contact our team so we can discuss the specific mutations required to maintain activity at your desired pH range.

Extended Services

Custom D-Tagatose Production Service

We specialize in bespoke D-tagatose production, leveraging cutting-edge biotechnology and proprietary enzyme engineering to deliver high-purity, cost-effective D-tagatose tailored to your specific industrial or research needs.

Glycan & Glycoprotein Structure & Profiling Services

Our comprehensive glycan and glycoprotein structure and profiling services employ state-of-the-art analytical techniques to provide in-depth insights into the complex carbohydrate structures and their functional roles.

Creative Biolabs provides an end-to-end custom DTEase production service that combines cutting-edge AI protein design with robust industrial fermentation. From increasing conversion rates to 85% to ensuring GRAS-certified safety, we empower your R&D to lead the rare sugar market. For detailed project discussions, technical consultations, or to receive a formal quotation, please reach out to our specialist team.

Reference

Miao, Peiyu, et al. "Advances and prospects of D-tagatose production based on a biocatalytic isomerization pathway." Catalysts 13.11 (2023): 1437. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/catal13111437.

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