D-ribulose-5-phosphate 3-epimerase is a vital Glycosyl Functional Enzyme in the pentose phosphate pathway (PPP), catalyzing the conversion between D-ribulose-5-phosphate and D-xylulose-5-phosphate. This reaction is essential for carbon flux regulation and the synthesis of key biomolecules like nucleotides and vitamins. The Epimerase supports energy metabolism, antioxidant defense, and cellular homeostasis. In biotechnology, it holds promise for metabolic engineering to enhance the production of biofuels, pharmaceuticals, and nutraceuticals.

Fig. 1. Homodimerization of CrRPI1. (OA Literature) Fig.1 CrRPI1 homodimerizatio.¹

Service Overview

Are you currently facing low product yields in the pentose phosphate pathway, difficulty in expressing stable TIM-barrel enzymes, or challenges in decoupling glucose/xylose co-fermentation? Our custom D-ribulose-5-phosphate 3-epimerase production service helps you achieve maximum metabolic efficiency and high-purity biocatalysts through our advanced recombinant expression platforms and structure-guided protein engineering. Creative Biolabs leverages these scientific insights to provide precision-engineered D-ribulose-5-phosphate 3-epimerase that ensures optimal carbon recycling for synthetic biology applications.

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D-Ribulose-5-Phosphate 3-Epimerase Production Workflow

Step 1

Gene Synthesis & Codon Optimization

We analyze the D-ribulose-5-phosphate 3-epimerase sequence and optimize it for your chosen expression host (e.g., E. coli or P. pastoris) to ensure maximum protein yield.

Step 2

Vector Construction & Transformation

The optimized gene is cloned into proprietary high-expression vectors and transformed into our validated chassis strains.

Step 3

Pilot Expression & Solubility Screening

Small-scale fermentation is conducted to evaluate protein solubility and enzymatic activity, ensuring the TIM-barrel structure is correctly folded.

Step 4

Fermentation Scale-up

Using state-of-the-art bioreactors, we scale production while monitoring parameters like dissolved oxygen and pH to maintain consistent enzyme quality.

Step 5

Purification & Quality Control

Multi-step chromatography (e.g., His-tag, ion exchange) is used to reach the target purity, followed by activity validation and stability profiling.

Fig. 2. D-Ribulose-5-Phosphate 3-Epimerase production workflow. (Creative Biolabs AI) Fig.2 Simplified production flow chart.

Final Deliverables

Purified D-ribulose-5-phosphate 3-epimerase

Lyophilized powder or liquid formulation with documented activity units.

Comprehensive QC Report

Including SDS-PAGE, SEC-HPLC purity data, and kinetic parameters.

Certificate of Analysis (CoA)

Detailed batch-specific data.

Service Features

Optimized Expression Systems
Proprietary microbial platforms (E. coli, Pichia, Bacillus) are designed to prevent inclusion body formation.

Structure-Guided Engineering
In-house expertise in modifying Loop 6 dynamics to improve substrate affinity and turnover rates.

High Purity & Activity
Guaranteed purity up to 98% with specific activity levels validated by coupled enzymatic assays.

Scalable Production
Flexible fermentation volumes ranging from milligram R&D scales to multi-kilogram industrial batches.

Comprehensive Characterization
Every batch undergoes rigorous profiling, including thermal stability (Tm) and pH tolerance assessments.

Key Applications

Metabolic Engineering & Synthetic Biology

These fields are of paramount importance in fine-tuning the non-oxidative branch of the pentose phosphate pathway (PPP). Through precise genetic and metabolic modifications, they enable the strategic redirection of carbon flux towards desired biochemical pathways, thereby enhancing the production efficiency of valuable compounds and optimizing cellular metabolism for specific industrial applications.

Vitamin & Nutraceutical Production

By augmenting the supply of ribulose-5-phosphate, a pivotal intermediate, these technologies significantly bolster the commercial-scale synthesis of Vitamin B2. This not only ensures a more abundant and cost-effective source of this essential vitamin but also supports the development of innovative nutraceutical formulations with enhanced bioavailability and health benefits.

Biofuel Development

In the pursuit of sustainable biofuel solutions, these approaches play a crucial role in managing D-xylulose 5-phosphate levels, thereby facilitating the efficient co-fermentation of glucose and xylose. This capability is particularly advantageous in lignocellulosic ethanol production, where the simultaneous utilization of both sugars maximizes ethanol yields, reduces production costs, and minimizes environmental impact, paving the way for a greener and more economically viable biofuel industry.

Pharmaceutical Intermediates

Acting as versatile biocatalysts, these technologies are instrumental in the synthesis of specialized 5-carbon sugars and phosphorylated metabolites, which serve as key intermediates in the production of a wide array of pharmaceutical compounds. Their ability to catalyze complex biochemical reactions with high specificity and efficiency accelerates the drug discovery and development process, enabling the production of novel and potent therapeutics with improved safety profiles.

Nucleic Acid Synthesis

Furthermore, these advancements provide the essential precursors required for the de novo synthesis of nucleotides in cell-free systems, a cornerstone of modern biotechnology. By enabling the rapid and precise assembly of nucleic acids, they support a broad range of applications, including genetic engineering, gene therapy, diagnostics, and synthetic biology, thereby driving innovation and progress in the life sciences.

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Customer Reviews

D-Ribulose-5-Phosphate 3-Epimerase Enhancement
"Using custom D-ribulose-5-phosphate 3-epimerase from Creative Biolabs in our riboflavin research has significantly improved the availability of D-ribulose-5-phosphate, leading to unprecedented yield stability."- 14 months ago, Dr. Al***son J.

Remarkable Stability
"The D-ribulose-5-phosphate 3-epimerase variants produced by Creative Biolabs showed remarkable stability at 45°C, which was critical for our high-temperature biocatalysis process compared to standard off-the-shelf enzymes."- 8 months ago, Dr. Mar*** H.

FAQs

Q: What is the purity level of the custom D-ribulose-5-phosphate 3-epimerase produced?

A: We typically offer purity levels of >90%, >95%, or >98%, depending on your application. We encourage you to contact us for specific requirements.

Q: Can you produce D-ribulose-5-phosphate 3-epimerase from non-standard organisms?

A: Yes, we specialize in codon optimization and expression of enzymes from extremophiles, plants, and rare microbial sources.

Q: How is the enzymatic activity verified?

A: We use a coupled enzyme assay involving transketolase or direct HPLC-based metabolite tracking to ensure the enzyme meets your specified activity units.

Q: Do you offer bulk quantities for industrial use?

A: Absolutely. Our fermentation capacity scales from pilot liters to industrial-scale volumes to support your commercial production.

Q: Are there precautions for enzyme storage?

A: Our D-ribulose-5-phosphate 3-epimerase is generally stable when stored at -80 °C with cryoprotectants, but we provide specific formulation services to enhance shelf-life at 4 °C for active use.

Extended Services

Custom D-Xylulose-5-Phosphoric Acid Production Service

Our tailored service offers bespoke production of D-xylulose-5-phosphoric acid, ensuring premium quality, precise specifications, and timely delivery to support your unique biochemical applications and research endeavors.

Epimerase Development & Production Service

We specialize in the cutting-edge development of high-performance epimerases, meticulously engineered to meet diverse industrial and research demands with unparalleled efficiency and specificity.

How to Contact Us?

Creative Biolabs provides a comprehensive suite of services for the custom production, engineering, and profiling of D-ribulose-5-phosphate 3-epimerase. From initial gene synthesis to large-scale industrial fermentation, we ensure your metabolic engineering projects are supported by high-quality, high-activity biocatalysts. For detailed project discussions or to request a customized quote, please reach out to our technical support team.

Reference

Le Moigne, Théo, et al. "High-resolution crystal structure of chloroplastic ribose-5-phosphate isomerase from Chlamydomonas reinhardtii-an enzyme involved in the photosynthetic Calvin-Benson cycle." International journal of molecular sciences 21.20 (2020): 7787. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms21207787.

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