GlycoErase™ ALG10 Knockout HepG2 Cell Line

The ALG10 knockout HepG2 cell line is a vital model for dissecting N-glycosylation's role in hepatocyte function and liver development, given HepG2's origin from a hepatoblastoma. ALG10 deficiency impairs N-glycosylation, potentially disrupting the folding and function of crucial hepatic proteins, including those involved in metabolism and detoxification. Researchers can use this model to investigate how N-glycosylation defects contribute to hepatoblastoma progression, chemoresistance, and to identify liver-specific diagnostic markers and therapeutic targets related to glycosylation pathways.

KO Cell Lines

Human

Epithelial-like, adherent

1:4~1:6

HepG2

Hepatoblastoma

Liver

Hepatoblastoma

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

DMEM & FBS & Glutamine

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALG10

ALG10 alpha-1,2-glucosyltransferase

ALG10A, DIE2, FLJ14751

12p11.1

This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.