GlycoErase™ ALG10 Knockout Human Ovarian Cancer Cell A2780

The ALG10 deficient human ovarian cancer cell line A2780 serves as a crucial in vitro system for dissecting the intricacies of N-glycosylation, where the absence of functional ALG10 leads to compromised N-glycan biosynthesis and consequential protein folding aberrations, thus enabling researchers to probe the involvement of N-glycosylation deficiencies in ovarian carcinoma characteristics such as proliferation, metastatic potential, and chemoresistance, alongside the identification of potential diagnostic indicators and therapeutic intervention points.

KO Cell Lines

Human

Epithelial-like, adherent

1:3~1:6

A2780

Ovarian Epithelial Tumor

Ovary/Fallopian Tube

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Glutamine & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALG10

ALG10 alpha-1,2-glucosyltransferase

ALG10A, DIE2, FLJ14751

12p11.1

This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.