GlycoErase™ ALG10 Knockout KD Cell Line

The ALG10 knockdown KD cell line, derived from an extra-cranial rhabdoid tumor, serves as a vital model for N-glycosylation research in this aggressive pediatric cancer. Exhibiting reduced expression of ALG10, these cells display impaired N-glycosylation, consequently affecting protein folding. Researchers utilize this ALG10-KD Rhabdoid Cancer cell line to investigate the impact of N-glycosylation defects on key aspects of rhabdoid tumor biology, such as proliferation, metastasis, drug resistance, and the evasion of immune surveillance, and to identify potential diagnostic markers or therapeutic targets specific to this challenging disease.

KO Cell Lines

Human

Epithelial-like, adherent

1:2~1:4

KD cell line

Rhabdoid cancer

Soft Tissue

Rhabdoid cancer

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

F-12 & FBS & Penicillin/Streptomycin

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ALG10

ALG10 alpha-1,2-glucosyltransferase

ALG10A, DIE2, FLJ14751

12p11.1

This gene encodes a membrane-associated protein that plays a critical role in N-linked glycosylation by adding the third glucose residue to the lipid-linked oligosaccharide precursor. Specifically, it transfers the terminal glucose molecule from dolichyl phosphate glucose (Dol-P-Glc) to the lipid-linked oligosaccharide Glc₂Man₉GlcNAc₂-PP-Dol. This step is essential for the proper assembly of the oligosaccharide precursor necessary for N-linked glycoprotein synthesis.