GlycoErase™ HEK293T B3GLCT Knockout Cell Line (CAT#: GLJF-0425-JF499)

Description	Researchers investigating Peters-plus syndrome (PPS) and O-linked glycosylation of thrombospondin type-1 repeats (TSRs) will find the B3GLCT knockout HEK293T cell line to be an invaluable resource. This cell line, lacking the B3GLCT-encoded beta-3-glucosyltransferase, enables investigations into the functional consequences of disrupting TSR glycosylation and its potential contribution to the development of PPS. The availability of this knockout in HEK293T cells, a platform widely used for gene function and regulation studies, further enhances its utility for researchers.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	B3GLCT
Full Name	Beta 3-glucosyltransferase
Alternative Name	B3Glc-T, B3GTL, B3GALTL
Location	13q12.3
Gene ID	145173
Summary	Beta-1,3-glucosyltransferase that transfers glucose to O-linked fucosylglycans on thrombospondin type-1 repeats; defects cause Peters-plus syndrome.