GlycoErase™ HEK293T B3GNT2 Knockout Cell Line (CAT#: GLJF-0425-JF500)

Description	We are pleased to introduce a B3GNT2 knockout HEK293T cell line, specifically designed to aid in the study of poly-N-acetyllactosamine chain biosynthesis. This cell line, deficient in the B3GNT2-encoded UDP-GlcNAc:betaGal beta-1,3-O-acetylglucosaminyltransferase 2, provides a unique opportunity to study the role of this enzyme in the synthesis of these complex glycan structures. Leveraging the versatility of HEK293T cells as a platform for gene function and regulation studies, researchers can utilize this model to gain deeper insights into the complexities of glycosylation.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:4
Cell Line	HEK293T
Lineage	Embryonic kidney

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Penicillin/Streptomycin
Application	Functional assay
Size	2 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	B3GNT2
Full Name	UDP-GlcNAc:BetaGal Beta-1,3-O-acetylglucosaminyltransferase 2
Alternative Name	B3GNT1, BETA3GNT, B3GN-T1, B3GNT-2, B3GN-T2
Location	2p15
Gene ID	10678
Summary	Type II transmembrane protein that prefers lacto-N-neotetraose as a substrate and participates in the biosynthesis of poly-N-acetyllactosamine chains.