GlycoErase™ LDHA Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF646)

Description	GlycoErase™ LDHA knockout HepG2 cell line removes LDHA expression, encoding the A subunit of lactate dehydrogenase, which catalyzes the conversion between pyruvate and lactate during anaerobic glycolysis. Mutations are associated with exertional myoglobinuria. This knockout enables research into glycolytic flux, liver energy metabolism, and lactate-associated pathophysiology, with applications in metabolic and cancer research.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	LDHA
Full Name	Lactate Dehydrogenase A
Alternative Name	LDHM; GSD11; PIG19; HEL-S-133P
Location	11p15.1
Gene ID	3939
Summary	This gene encodes the A chain of lactate dehydrogenase, which catalyzes the reversible conversion of pyruvate to lactate with concurrent oxidation of NADH to NAD during anaerobic glycolysis. Predominantly expressed in skeletal muscle, it belongs to the lactate dehydrogenase family. Mutations have been linked to exertional myoglobinuria. Several nonfunctional pseudogenes exist in the human genome.