GlycoErase™ SIGLEC14 Knockout HepG2 Cell Line (CAT#: GLJF-0825-JF866)

Description	GlycoErase™ SIGLEC14 knockout HepG2 cell line is a HepG2 model in which SIGLEC14 expression has been eliminated. SIGLEC14 is predicted to enable sialic acid binding and cell adhesion, and localize to granule membranes and the plasma membrane. The knockout HepG2 line provides a valuable system to investigate adhesion biology, innate immune activity, and membrane receptor function, with applications in hepatocellular carcinoma research, viral biology, and molecular drug studies.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:4~1:6
Cell Line	HepG2
Primary Disease	Hepatoblastoma
Lineage	Liver
Lineage Subtype	Hepatoblastoma

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	DMEM & FBS & Glutamine
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC14
Full Name	Sialic Acid Binding Ig Like Lectin 14
Alternative Name	Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	100049587
Summary	This protein is predicted to bind sialic acid and is involved in cell adhesion. It is predicted to be located in ficolin-1-rich granule membranes and active within the plasma membrane.