GlycoFlux™ Human SIGLEC14 Overexpression A549 Cell Line (CAT#: GLJF-0925-JF862)

Description	GlycoFlux™ human SIGLEC14 overexpression A549 cell line is a stable epithelial model engineered to express SIGLEC14, a predicted sialic acid-binding receptor associated with innate immune recognition. Localized to the plasma membrane, ficolin-1-rich granule membranes, and tertiary granule membranes, it functions in cell adhesion and immune modulation. This model offers a tractable system for investigating epithelial innate responses, lectin-mediated adhesion, and glycan-dependent regulation of pathogen defense.
Product Type	Overexpression Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:3~1:6
Cell Line	A549
Primary Disease	Non-small cell lung cancer
Lineage	Lung
Lineage Subtype	Non-small cell lung cancer

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	qPCR, Sanger Sequencing
Culture Medium	F-12K & FBS & Penicillin/Streptomycin
Application	Mechanistic studies; Exploration of glycosylation and signaling pathways in cancer, metabolic, and immune-related diseases; Drug target validation and other functional assays.
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	SIGLEC14
Full Name	Sialic Acid Binding Ig Like Lectin 14
Alternative Name	Sialic Acid Binding Ig Like Lectin
Location	19q13.41
Gene ID	100049587
Summary	Predicted to enable sialic acid binding activity. Predicted to be involved in cell adhesion. Predicted to be located in ficolin-1-rich granule membrane and tertiary granule membrane. Predicted to be active in plasma membrane.