GlycoErase™ ST6GAL1 Knockout CHO-K1 Cell Line

The ST6GAL1 knockout CHO-K1 cell line, utilizing gene editing technology to precisely knockout ST6 beta-galactoside alpha-2,6-sialyltransferase 1 gene, glyco-engineering analysis shows that this cell line may lead to severe dysfunction of the critical α-2,6-sialylation modification pathway in CHO cells, because ST6GAL1 protein specifically catalyzes the linkage of α-2,6-sialic acid, directly determining the terminal sialylation modification level and final product quality of therapeutic glycoprotein drugs.

KO Cell Lines

Hamster

Epithelial, adherent

1:3~1:5

CHO-K1

Chinese hamster ovary

>90%

The sterility test indicated an absence of microbial growth.

STR identification

Negative

Negative for HIV, HBV and HCV.

We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.

PCR, Sanger Sequencing

FBS & Penicillin/Streptomycin & Proline & RPMI

Functional assay

1 M cells/vial*2

Frozen

Dry ice

Made to order

Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.

This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

ST6GAL1

ST6 Beta-galactoside alpha-2,6-sialyltransferase 1

SIAT1, CDw75

3q27.3

Transfers sialic acid to galactose-containing substrates, involved in the generation of cell-surface carbohydrate determinants and differentiation antigens.