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The GlycoSens™ Adenosine Monophosphate (AMP) Assay Kit provides an advanced, quantitative high-performance liquid chromatography (HPLC) method for determining AMP in biological specimens. Featuring superior kinetic linearity and high throughput compatibility, it facilitates accurate biochemical profiling.
Adenosine monophosphate (AMP) exhibits a strong UV absorption peak at 254 nm. Quantitative determination of AMP concentration is performed using high-performance liquid chromatography (HPLC).
Detection Time
Within 1 hour
Detection Wavelength
254 nm
Required Equipment & Materials (Self-provided by User)
Known for its specific biological characteristics, adenosine monophosphate (AMP) provides deep insights into cellular dynamics. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Optimized for high-throughput profiling of adenosine monophosphate (AMP) in tissue bioenergetics profiling, cofactor regeneration mechanics, and biochemical pathway modeling.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.