GlycoSens™ Caspase-3 Assay Kit

The GlycoSens™ Caspase-3 Assay Kit features an optimized micro-volume detection system designed for sensitive measurement of caspase-3 levels across diverse research samples. The assay components are ready-to-use, ensuring high analytical reproducibility with minimal sample requirements.

Basal Metabolic Series

Caspase-3

Extraction and assay buffer; Substrate Mix A; Substrate Mix B; Reaction developer

Tissues, other biological samples

10 μL - 50 μL

Quantification of caspase-3 is established through a specific substrate-mediated reaction. Signal intensity recorded via microplate reader or spectrophotometer is proportionally calibrated against analytical standard curves.

Within 2 hours

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Caspase-3

Caspase-3

Known for its specific biological characteristics, caspase-3 provides deep insights into cellular dynamics. Its concentration levels are highly indicative of the functional state and metabolic vigor of the sample. Moreover, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.

Widely applicable for tracking caspase-3 fluctuations across agricultural biotechnology, crop growth regulation, and physiological stress responses.

Rapid Execution; Flexible Sample Inputs; Highly Reproducible Data

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.