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The GlycoSens™ Caspase-3 Assay Kit provides a robust, high-precision analytical assay platform engineered for quantification of target caspase-3 in varied biological matrices. Featuring a streamlined protocol, it delivers outstanding linearity and operational speed for routine biochemical assays.
Product Type
Other Series
Target
Caspase-3
Kit Components
Extraction Buffer; Reaction Reagent A; Reaction Reagent B; Standard
Sample Types
Tissues, other biological samples
Sample Volume
20 μL - 100 μL
Detection Principle
The assay relies on the distinct spectroscopic optical characteristics of caspase-3. Upon reaction with specialized chromogenic reagents, the resulting optical density shifts measured at target wavelengths directly reflect caspase-3 content in the sample.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Caspase-3
Alternate Names
Caspase-3
Application Notes
Background
Research highlights caspase-3 as a significant participant in key metabolic and regulatory cascades. It actively circulates within the biological matrix, acting as a crucial building block or signaling molecule. Furthermore, thus, accurate determination using this assay supports advanced exploration in biochemistry and molecular biology.
Application
Optimized for high-throughput profiling of caspase-3 in functional metabolomics, secondary metabolite profiling, and environmental adaptation research.
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.