GlycoSens™ Choline Acetyltransferase (ChAT) Assay Kit

The GlycoSens™ Choline Acetyltransferase (ChAT) Assay Kit offers an advanced quantitative methodology for evaluating choline acetyltransferase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.

Fatty Acid Series

ChAT

Sample Lysis Buffer; Substrate; Detection Reagent; Standard

Tissues, other biological samples

10 μL - 50 μL

The system measures chromatic product formation derived from target choline acetyltransferase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of ChAT levels.

Within 45 minutes

Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.

Store at -20°C.

Shipped with ice packs.

Choline Acetyltransferase

Choline Acetyltransferase; ChAT

Functioning at the core of various biological processes, choline acetyltransferase (ChAT) is a key subject of biochemical analysis. This catalytic protein strictly regulates substrate turnover to meet the dynamic physiological needs of the cell. Notably, evaluating its levels provides researchers with invaluable insights into metabolic alterations and health status.

Tailored for accurate quantification of choline acetyltransferase (ChAT) across cell biology, organelle bioenergetics, and intracellular signaling cascades.

Rapid Reaction Protocol; Stringent Quality Control; Enhanced Sensitivity

1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.

2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.

3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.