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The GlycoSens™ Citrate Synthase (CS) Assay Kit offers an advanced quantitative methodology for evaluating citrate synthase concentration with high specificity. Designed to minimize matrix background interference, this kit facilitates high-throughput scientific profiling and consistent experimental turnaround.
Product Type
Tricarboxylic Acid Cycle Series
Target
CS
Kit Components
Sample Lysis Buffer; Substrate; Detection Reagent; Standard
Sample Types
Tissues, other biological samples
Sample Volume
50 μL
Detection Principle
The system measures chromatic product formation derived from target citrate synthase activity. Absorbance changes tracked at optimal wavelengths allow precise quantitative determination of CS levels.
Detection Time
Within 2 hours
Required Equipment & Materials (Self-provided by User)
Visible Spectrophotometer or Microplate Reader, glass/quartz cuvettes, microcentrifuge, water bath/incubator, adjustable micropipettes, double-distilled water.
Storage
Store at -20°C.
Shipping Conditions
Shipped with ice packs.
Target Information
Full Name
Citrate Synthase
Alternate Names
Citrate Synthase; CS
Application Notes
Background
Citrate synthase (CS) acts as an essential biochemical entity within diverse cellular pathways. This enzyme drives anabolic pathways, enabling the targeted assembly of essential structural or metabolic compounds. Furthermore, assessing this component offers a reliable parameter for agricultural research or biological studies.
Application
Ideal for investigating citrate synthase (CS) activity and metabolic turnover during natural product research, growth factor signaling, and developmental morphogenesis studies.
Key Features & Advantages
High Specificity; Stable Reaction; Broad Sample Compatibility
Operating Instructions
1. Pre-Centrifugation of Vials: Prior to opening any reagent vials, perform a brief centrifugation to collect all powder or liquids at the bottom of the tubes.
2. Reagent Equilibration & Mixing: Allow all kit reagents and buffers to equilibrate to room temperature (25 °C) before initiating the assay.
3. Sample Storage: If samples are not assayed immediately, please store at -80 °C and avoid repeated freeze-thaw cycles.